Anti-Prostatic Acid Phosphatase (Prostatic Acid Phosphotase, PAP, Acid Phosphatase Prostate, ACPP, A

ACPP is an ~50kD glycoprotein member of the histidine acid phosphatase family of enzymes. It exists as a 95-100kD nondisulfide-linked homodimer that hydrolyzes phosphate esters under low pH to generate free phosphate. ACPP is expressed by prostate epithelium and pain-detecting spinal cord neurons. In the spinal cord, ACPP dephosphorylates AMP. This generates adenosine which acts as a strong analgesic agent. Mature human ACPP is 354aa in length (aa33-386). It contains one histidine phosphatase domain (aa34-332), plus a nucleophile acceptor site at His44, and a proton donor site at Asp290. There are two potential alternative splice variants. One shows a deletion of aa153-185, while another is transmembrane (previously called TMPase) and shows a 38aa substitution for the C-terminal seven aa. Over aa33-379, human ACPP shares 84aa identity with mouse ACPP. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml, Immunohistochemistry: 8-25ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.