Anti-Plastin L (PLSL, L Plastin, L-Plastin, LC64P, Lymphocyte Cytosolic Protein 1, LCP1, LCP-1, Plas

Highly conserved and widely expressed plastin proteins comprise a subset of actin-binding proteins that include proteins that promote actin bundling. Three plastins exhibiting differential expression are found in mam-mals and include L-plastin, T-plastin and I-plastin. T-plastin (plastin-3) is found in cells of most solid tissues while I- plastin (plastin-1) is expressed specifically in kidney, colon and small intestine (1-3). L-plastin (plastin-2) or LCP1 (lymphocyte cytosolic protein 1) is mainly expressed in hematopoietic cells and non-hematopoietic tumors, including increased expression correlated with metastatic progression in colon cancer cell lines (4). Overexpression of LCP1 in premetastatic cancer cell lines induces invasion and loss of E-cadherin expression, which is characteristic of metastatic cancer cell lines (5). LCP1 becomes phosphorylated at Ser5 upon stimulation through the T-cell receptor/co-receptor complex (TCR/CD3) in association with the CD2 cell adhe-sion molecule or the CD28 receptor (6). Phosphorylation at Ser5 increases LCP1 ability to bind F-actin and increases cell motility (7,8). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. , Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.