Anti-PI 3-Kinase p85 alpha (Phosphatidylinositol-PI-3-Kinase)

PI 3-Kinases phosphorylate the inositol ring on a variety of membrane-associated phosphatidylinositols. Class I phosphoinositide 3-kinases (PI 3-Kinases) are heterodimeric proteins that consist of a catalytic subunit of 110-120kD and an associated regulatory subunit. p85a is one of the regulatory subunits that associate with the class IA PI 3-Kinases. Composed of one SH3 and two SH2 domains, p85a functions as an adaptor, coupling catalytic p110 to activated receptor tyrosine kinases. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml, Optimal dilutions to be determined by the researcher. Hybridoma: Mouse myeloma cells with B cells obtained from a mouse immunized with purified, E. coli-derived, recombinant human PI 3-Kinase p85a (aa519-644). Storage and Stability: Lyophilized powder may be stored at -20°C for short-term only. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.