Anti-Phospholipase C beta2

Phospholipase C beta2 (PLCbeta2) is an enzyme that catalyzes the hydrolysis of phosphatidylinositol 4,5-bisphosphate to the secondary messengers inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). It is approximately 135 kDa and is composed of a pleckstrin homology domain, four EF-hand motifs, a catalytic domain, and a C2 domain that are common to all PLCs, as well as a 400-amino acid C-terminal region that is required for PLCbeta2 activation by the G-protein subunits Galphaq or Galpha11. PLCbeta2 is also activated by Gbetagamma subunits. Upon activation by a G-protein subunit, PLCbeta2 initiates intracellular signal transduction of extracellular signals using calcium as a cofactor. PLCbeta2 colocalizes with T1R and T2R taste receptors and Plcb2-/- mice exhibit selective and complete loss of sweet, amino acid, and bitter tastes. Plcb2-/- mice also exhibit increased coxsackievirus A16-induced pro-inflammatory cytokine production and decreased survival compared with wild-type mice. Cayman's Phospholipase C beta2 Polyclonal Antibody can be used for immunohistochemistry (IHC) and immunofluorescence (IF) applications.Synonyms: 1-Phosphatidylinositol 4,5-bisphosphate Phosphodiesterase beta2, PLCbeta2, Phosphoinositide Phospholipase C beta2. Immunogen: Peptide from the C-terminal region of human PLCbeta2. MW: ~135 kDa. Formulation: (Request formulation change), 100 µl of affinity-purified polyclonal antibody from pooled serum. Storage Buffer: 10 mM HEPES, pH 7.5, with 150 mM sodium chloride, 100 µg/ml BSA, and 50% glycerol. Host: Rabbit. Applications: IHC, IF.