Anti-PGPEP1 (Pyroglutamyl-peptidase 1, 5-oxoprolyl-peptidase, Pyroglutamyl Aminopeptidase I, PAP-I,

Pyroglutamyl peptidase-1 is an omega peptidase which removes pyroglutamyl residues from the amino termini of peptides and proteins. It is a cytosolic cysteine peptidase that is expressed in most cell types. The enzyme requires a thiol-reducing agent for activity. PGPEP-1 is potentially involved in the inactivation of biologically active peptides that possess an a terminal pyroglutamyl group. Examples of such peptides include neurotensin, luteinizing hormone releasing hormone, and thyrotropin-releasing hormone. Human and mouse PGPEP-1 share 95%aa sequence identity. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.