Anti-PACAP-Related Peptide (Pituitary Adenylate Cyclase Activating Polypeptide Related Peptide)

Pituitary adenylate cyclase activating peptide (PACAP), originally isolated from ovine, hypothalamus, belongs to the VIP-family of peptides. PACAP-related peptide (PRP) is a 29aa peptide, which is produced together with PACAP-27 and PACAP-38 when Prepro PACAP is being processed. PRP is abundant in the brain, but can be also found in the respiratory and gastrointestinal tracts. Absorption with 10-100ug immunogen per ml diluted antiserum abolishes the staining, while PACAP-27, PACAP-38, VIP, PHI, CRF, oxytocin and vasopressin do not. Applications: Suitable for use in Immunofluorescence microscopy (frozen sections). Recommended Dilution: Immunofluorescence microscopy: 1:400-1:800 with overnight incubation at 2-8°C. Positive Control: Stefanini-fixed frozen sections of rat duodenum or hypothalamus. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.