Anti-NudE-L (NudE-like Protein, Nudel, Nuclear Distribution Gene E Like Homolog 1, NDEL1, DKFZp451M0

Nudel, or endooligopeptidase A, a protein highly expressed in the brain where it associates with dynein motors and Lis1, both of which have been linked to neuronal development and migration. Nudel facilitates the polymerisation of neurofilaments and the organisation of the cellular microtubule array and microtubule anchoring at the centrosome. It is required for several dynein- and microtubule-dependent processes such as the centripetal motion of secretory vesicles, coupling of the nucleus and centrosome and maintaining Golgi integrity. Nudel also serves as a Cdk5 substrate and its phosphorylation status affects its localisation in neurons. Applications: Suitable for use in ELISA and Western Blot. Other Applications not tested. Recommended Dilutions: ELISA: 1:8000, Western Blot: 0.1-0.3ug/ml, Optimal dilutions to be determined by the researcher, Positive Control: Human Brain (Cerebellum) lysates, Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 monthsat -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Peptide Blocking: Corresponding peptide is available for Peptide Blocking studies. See N7400-30D-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.