Anti-Ninein (Glycogen Synthase Kinase 3 beta-interacting Protein, GSK3B-interacting Protein)

Ninein (UniProt Q61043, also known as Glycogen synthase kinase 3 beta-interacting protein, GSK3B-interacting protein) is encoded by the Nin (also known as Kiaa1565) gene (Gene ID 18080) in murine species. Ninein is a microtubule (MT) minus-end-binding protein that helps anchor the MTs to the centrosome. Ninein is also found in noncentrosomal locations in association with the minus-end of noncentrosomal MTs present in polarized cells. Ninein is essential for the self-renewal and maintenance of apical progenitors (APs) in developing mouse neocortex by anchoring of MTs to the centrosome, while only noncentrosomal ninein is seen in cortical neurons where it is localized to the axons and the somatodendritic compartment. Centrosome-associated ninein plays an important role in stabilizing the radial MT asters, while noncentrosomal ninein plays an MT-stabilizing role in the axons of neurons. Ninein is a downstream target of the transcription factor Sip1 involved in CNS development. Re-expression of ninein in Sip1-deficient neurons or overexpressing ninein in wild-type neurons significantly increases their resistance to MT depolymerization. Applications: Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 1:250 detects cytoplasmic ninein immunoreactivity in human brain and spleen tissue sections. , Immunocytochemistry: 1:500 detects cytoplasmic and centrosome ninein immunoreactivity in 0.1 Triton X-100 extracted and 4% paraformaldehyde-fixed HEK293T cells, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.