Anti-NHE6 (Na, H Exchanger 6)

Na+/H+ exchangers (NHE) of mammalian cells are plasma membrane intrinsic proteins mediating exchange of N+ and H+ ions in various tissues. The NHE catalyzes the electroneural transport of extracellular Na+ for intracellular H+. They play a major role in regulation of intracellular pH (pHi) in addition to trans-cellular absorption of Na+, cell volume regulation and possibly in cell proliferation. These primary functions of the Na+/H+ exchanger have been related to many pathophysiological states, include hypertension, organ growth and hypertrophy, regression of cancer and renal intestinal disorders. At least 7 NHE isoforms (NHE1-7) have been cloned so far. They are all similar in their primary structure and predicted to have 10-12 transmembrane domains. The C-terminal domain of NHEs are predicted to be intracellular., A mammalian homolog of yeast NHA2 has been identified and classified as NHE6 (KIAA0267). NHE6 is 669 aa (mol wt ~75 kD), and predicted to contain 12 TM domains within the cytoplasmic N-terminus, the C-terminus may reside in the matrix. NHE6 is ubiquitously expressed but it is most abundant in mitochondrial-rich tissues such as brain, skeletal muscle, and heart. Applications: Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 1:100,000 using 50-100ng control peptide/well. , Western Blot: 1-10ug/ml using Chemiluminescence. NHE6 has a calculated mol wt of ~75kD., Optimal dilutions to be determined by the researcher. Recommended Control Peptide: N2020-60: NHE6, Human (Na, H Exchanger 6, KIAA0267), Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.