Anti-NHE3 (Na, H Exchanger 3)

Na+/H+ exchangers (NHE) of mammalian cells are plasma membrane intrinsic proteins mediating exchange of N+ and H+ ions in various tissues. The NHE catalyzes the electroneural transport of extracellular Na+ for intracellular H+. They play a major role in regulation of intracellular pH (pHi) in addition to trans-cellular absorption of Na+, cell volume regulation and possibly in cell proliferation. These primary functions of the Na+/H+ exchanger have been related to many pathophysiological states, include hypertension, organ growth and hypertrophy, regression of cancer and renal intestinal disorders. Seven NHE isoforms (NHE1-7) have been cloned so far. They are all similar in their primary structure and predicted to have 10-12 transmembrane domains. The COOH-terminals of NHE1, NHE2 and NHE3 are intracellular. NHE3 (rat, 831aa, human chromosome 5 p15.3) is involved in trans-epithelial Na+ absorption. The NHE3 mRNA is found in kidney cortex, medulla, jejunum, ileum, colon and stomach (1). , Applications: , Suitable for use in ELISA, Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000 using ECL. A band of 95-110kD has been detected in brush border membrane (2)., ELISA: 1:100,000 Control peptide can be used to coat ELISA plates at 1ug/ml. Optimal dilutions to be determined by the researcher. , Control Peptide: N2017-29, Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.