Anti-NF-kappaB (p65)

NF-kappaB p65 is a ubiquitously expressed transcription factor that is a subunit of the NF-kappaB complex and is encoded by the RELA gene in humans. It is composed of an N-terminal Rel homology domain, which mediates dimerization, nuclear localization, and DNA and protein interactions, and two C-terminal transactivation domains that are subject to a variety of post-translational modifications and regulate the transcriptional activity of p65. NF-kappaB p65 regulates the expression of a large number of genes in response to inflammatory and environmental cues that play critical roles in innate and adaptive immunity and cellular differentiation. Genome-wide deletion of Rela in mice is embryonic lethal. Silencing of NF-kappaB p65 induces tumor cell apoptosis in a murine Lewis lung carcinoma model and decreases secreted levels of IL-1beta and TNF-alpha induced by LPS in THP-1 monocytes. NF-kappaB p65 is overexpressed in inflamed joints of patients with rheumatoid arthritis, and naïve CD4 T cells isolated from the whole blood of patients with multiple sclerosis have increased phosphorylation of NF-kappaB p65. Cayman's NF-kappaB p65 (human, recombinant) protein can be used for Western blot and ELISA applications. Cayman's NF-kappaB (p65) Polyclonal Antibody can be used for ELISA and Western blot applications. The antibody recognizes NF-kappaB at 65 kDa from human samples.Synonyms: Nuclear Factor NF-kappaB (p65) subunit, Transcription Factor p65. Immunogen: Synthetic peptide from the C-Terminal region of human NF-kappaB (p65). Formulation: (Request formulation change), 500 µl of peptide affinity-purified polyclonal antibody. Storage Buffer: TBS, pH 7.4, with 50% glycerol, 0.1% BSA, and 0.02% sodium azide. Host: Rabbit. Isotype: IgG. Applications: ELISA and WB. UniProt Accession #: Q04206.