Anti-Neuron Specific Enolase (NSE, Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, Neural Enolase,

The glycolytic enzyme enolase (2-phospho-Dglycerate hydrolyase) exists as several dimeric isoenzymes (aa, ab, ag, bb and gg) composed of three distinct subunits: a, b, and g (alpha, beta, and gamma). Three isoenzymes are found in human brain: aa, ag, and gg. The ag and gg-enoloase isoenzymes are also known as neuron- specific enolase (NSE) as these isoenzymes initially were detected in neurons and neuronendocrine cells. Lung cancer is one of the most spread cancer forms with incidences about 50-100 per 100,000 population. Approximately 20% of the lung cancer is small cell lung cancer. NSE has been shown to be a valuable tumor marker of neuroendocrine origin, particulary in small cell lung cancer and in neuroblastoma. Patients with small cell lung cancer show various proportions of ag and gg isoenzymes. The determination of NSE should detect ag and gg isoforms with the same sensitivity. The antibodies for this particular assay are specific for the g-subunit without cross reactivity with a or b subunits. NSE is reported to be useful diagnostic marker for lung cancer, neuroblastoma, melnoma, seminoma and in injury of central nervous system. In addition to the above, NSE can be a valuable tool in following-up the effect of chemotherapy of small cell lung cancer, in prognostic evaluation of patients with small cell lung cancer, and in differential diagnosis between cell lung cancer and non-small cell lung cancer. Applications: Suitable for use in ELISA, Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:10,000-1:100,000, Western Blot: 1:1000-1:5000, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.