Anti-Nesprin 1 (CPG2, Enaptin, MYNE1, SCAR8, Synaptic Nuclear envelope Protein 1, Syne1, SYNE1B)

There are two genes encoding members of a new family of type II integral membrane proteins. Both are ubiquitously expressed and tissue-specific alternative mRNA initiation and splicing generate at least two major isoforms of each protein with the smaller isoforms being truncated at the N-terminus. These proteins are called Nesprin l and 2 for nuclear envelope spectrin repeat as they are characterized by the presence of multiple clustered spectrin repeats bipartite nuclear localization sequences and a conserved C-terminal single transmembrane domain. Transient transfection of EGFP-fusion expression constructs demonstrated their localization to the nuclear membrane with a novel C-terminal TM domain-containing sequence essential for perinuclear localization. Nesprin l is developmentally regulated in both smooth and skeletal muscle and is relocalized from the nuclear envelope to the nucleus and cytoplasm during C2Cl2 myoblast differentiation. Nesprins may function as dystrophins of the nucleus to maintain nuclear organization and structural integrity. There are two genes encoding members of a new family of type II integral membrane proteins. Both are ubiquitously expressed and tissue-specific alternative mRNA initiation and splicing generate at least two major isoforms of each protein with the smaller isoforms being truncated at the N-terminus. These proteins are called Nesprin l and 2 for nuclear envelope spectrin repeat as they are characterized by the presence of multiple clustered spectrin repeats bipartite nuclear localization sequences and a conserved C-terminal single transmembrane domain. Transient transfection of EGFP-fusion expression constructs demonstrated their localization to the nuclear membrane with a novel C-terminal TM-domain-containing sequence essential for perinuclear localization. Nesprin l is developmentally regulated in both smooth and skeletal muscle and is relocalized from the nuclear envelope to the nucleus and cytoplasm during C2Cl2 myoblast differentiation. Nesprins may function as dystrophins of the nucleus to maintain nuclear organization and structural integrity. Applications: Suitable for use in Immunofluorescence, Western Blot, Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunocytochemistry/Immunofluorescence: 1:200, Western Blot: 1:400-1:800. Predicted molecular weight: 1011kD, Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.