Anti-Nepmucin (CD300LG, a not expressed in Peyer's Patch mucin, CLM-9) (Biotin)

Nepmucin (a not expressed in Peyer's Patch mucin, also CLM-9) is an 80-95kD member of the CD300L family of proteins. It is expressed on endothelium and serves multiple functions. It is known to form homotypic interactions between endothelial cells, bind lymphocytes via its Ig-like and mucin domains and serve as a transcellular transport vehicle for IgA and IgM. Mature mouse Nepmucin is a 397 amino acid (aa) type I transmembrane glycoprotein. Its extracellular region (aa 19-288) contains one V-type Ig-like domain (aa 19-124) plus an O-glycosylated mucin region (aa 136-276). There are three isoform variants. One is 60-75kD in size and shows an Ala substitution for aa 126-170, a second is 46-50kD in size and shows an Arg substitution for aa 170-254, while a third is 36kD in size and shows a SerTyr substitution for aa 134-256. Over aa 1-284, mouse Nepmucin shares 89% and 53% aa identity with rat and human Nepmucin, respectively., , Applications:, Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect mouse Nepmucin. The detection limit for rmNepmucin is approximately 1ng/lane under non-reducing and reducing conditions. , Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.