Anti-Neomycin Phosphotransferase II (NPT II)

Neomycin phosphotransferase II confers resistance to kanamycin and neomycin in bacteria and G418 (Geneticin(R), G418 Sulfate) in mammalian cells. These antibiotics bind to ribosomal components and inhibit protein synthesis. NPT II inhibits these antibiotics through phosphorylation, which is thought to interfere with their active transport into the cell. Western Blot analysis of transfected cell lysates with anti-NPT II can be used to gauge relative transfection efficiencies in experiments where multiple transfections are performed in parallel with different plasmids. Applications: Suitable for use in ELISA and Western Blot. Other applications have not been tested. Recommended Dilutions: Western Blot: Detects NPT II in RIPA lysates from Cos-1 cells transfected with pUSE, harboring the neomycin resistance gene, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.