Anti-NALP2 (NACHT-, LRR- and PYD-Containing Protein 2, PYRIN-containing APAF1-like Protein 2, Nucleo

NALP2 (NAcht Leucine-rich repeat Protein 2, also known as PAN1) is a cytosolic, 121kD member of the NLRP family of proteins. It is expressed in macrophages and exhibits divergent effects on inflammation. Via NFkappaB, it suppresses TNF-alpha production in response to LPS, via procaspase-1, it promotes proIL-1Beta cleavage and release. Human NALP2 is 1062 amino acids (aa) in length. It contains an N-terminal Pyrin domain (aa 9-90), followed by a Nacht region and nine LRRs (aa 467-1033). Four alternate isoforms exist. Three show deletions of aa's 109-132, 133-154 and 847-1062, respectively, a fourth shows a 38 aa substitution for aa's 1-903. No close rodent counterparts have been reported. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1.0ug/ml., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.