Anti-NALP1 (NACHT-, LRR- and PYD-Containing Protein 1, DEFCAP, NAC, CARD7, Caspase Recruitment Domai

NALP1 is a 160-170kD member of the NLRP family of molecules. It is expressed in dendritic cells, neutrophils, T and B cells, alveolar macrophages, permatogonia, neurons, plus intestinal columnar epithelium, and is found in both cytoplasm and nucleus. NALP1 promotes apoptosis plus IL-1b and IL-18 maturation by activating caspase-1 and -5. It does so by forming a 700kD inflammasome comprised of NALP1, ASC plus caspase-1 and -5. Bacterial wall peptidoglycan binds to NALP1, promoting ATP binding, NALP1 oligomerization, and caspase activation. Human NALP1 is 1473aa in length. It contains an N-terminal DAPIN domain (aa1-92), a NACHT domain (aa328-637), seven consecutive LRRs (aa704-1236) and one CARD region (aa1374-1463). Alternate splice forms exist that range in size from 70-150kD. Either individually, or in combination, there can be a 19aa substitution for either aa1353-1473 or aa1368-1472, a deletion of aa91-260, 958-987, 1262-1305, and a 43aa insert after aa785. Over aa1-323, human NALP1 shares less than 20aa identity with mouse NALP1. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.