Anti-MUC-1 (EMA, Epithelial Membrane Antigen Mucin-1 )

In Western blotting, it recognizes proteins in MW range of 265-400kD, identified as different glycoforms of EMA. This monoclonal antibody reacts with the DTRP epitope in the tandem repeats. The alpha subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain, which is involved in cell signaling, through phosphorylation s and protein-protein interactions. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinoma tissues. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver. Applications: Suitable for use in ELISA, Flow Cytometry, Western Blot, Immunoprecipitation, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-1ug/10e6 cells, Western Blot: 0.5-1ug/ml, Immunofluorescence: 1-2ug/ml, Immunoprecipitation: 1-2ug/500ug protein lysate, Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT, Optimal dilutions to be determined by the researcher. Positive Control: MCF-7 or MDA-231 cells. Breast or colon carcinoma. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.