Anti-Msx1 (Msh Homeobox 1, Msh Homeobox Homolog 1, Msx-1, Homeobox Protein MSX-1, Homeobox Protein M

Msh homeobox 1 (Msx1) is a Muscle Seg-ment Homeobox (Msh) gene family member that acts as a transcriptional repressor during embryonic development, playing an important role in limb pattern formation, cranio-facial development, and tooth development (1-3). Msx1 is , expressed in the mesenchyme of the developing nail bed (2) and in fetal hair follicles, epidermis and fibroblasts, reduced expression is seen in adult epithelial-derived tissues (4). , Msx1 acts in concert with the Wnt1 network to establish the midbrain dopaminergic progenator domain, a region that gives rise to neurons that are critical for normal brain function and are the cells affected in Parkinson disease (5). Mutation in the corresponding Msx1 gene correlates with , abnormal tooth development in patients diagnosed with Wolf-Hirschhorn syndrome (6). Other genetic changes in the Msx1 gene result in Witkop Syndrome ("tooth and nail syndrome") and cases of abnormal tooth development as-sociated with non-syndromic orofacial clefting (2,7). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. , Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.