Anti-Mouse Breast Cancer Resistance Protein, clone BXP-53 (Monoclonal) [Rat]

MON9051C was selected after immunization with a fusion protein containing the E. coli maltose binding protein and a fragment of the mouse bcrp protein corresponding to amino acids 221-394. BXP-53 reacts with an internal epitope of bcrp, a 70 kD transmembrane half-transporter which is involved in Multidrug resistance. BXP-53 also reacts with the human BCRP molecule. M6II-31 can be used to detect human MRP6 in cells and tissues. Immunocytochemistry: use 1:20-50 dilution on acetone fixed cytospin preparations. For immunohistochemistry: M6II-31 (use 1:20-50) on acetone fixed frozen sections can be followed by incubation with rabbit anti-rat IgG (1:25, Dako) and an APAAP complex (1:50, Dako). M6II-31 can be used on formaldehyde-fixed paraffin-embedded human tissues, after pretreatment with 0.01 M citric acid (pH 6.0) in distilled water at 100 C for 10 minutes. After incubation of M6II-31 (use 1:20) and washing, slides can be incubated with biotinylated rabbit anti-rat IgG (1:100, Jackson, West Grove) and streptavidin conjugated to horseradish peroxidase (1:500, Zymed, San Francisco, CA). Flow cytometry: optimal conditions still to be defined. Western blotting: use 1:20-50 dilution and anti-rat-HRP. Protein function: [Isoform 1]: May participate directly in the active transport of drugs into subcellular organelles or influence drug distribution indirectly. Transports glutathione conjugates as leukotriene-c4 (LTC4) and N-ethylmaleimide S-glutathione (NEM-GS). [The UniProt Consortium]