Anti-Malate Dehydrogenase (MDH) (HRP)

Anti-Malate Dehydrogenase (MDH) (HRP)
Artikelnummer Größe Datenblatt Manual SDB Lieferzeit Menge Preis
M2115-20.100 100 µg - -

3 - 19 Werktage*

916,00 €
 
Malate dehydrogenase (MDH) catalyzes the interconversion of L-malate and oxaloacetate using... mehr
Produktinformationen "Anti-Malate Dehydrogenase (MDH) (HRP)"
Malate dehydrogenase (MDH) catalyzes the interconversion of L-malate and oxaloacetate using nicotinamide adenine dinucleotide (NAD) as a coenzyme. MDH is found in all eukaryotic cells as two isozymes: mitochondrial (m-MDH) and cytoplasmic (soluble, s-MDH). Prokaryotes contain only a single form. The two isozymes, both consisting of two very similar subunits of about 35kD and having similar enzymatic activity appear as different proteins (Bleile, et al., 1975). Noyes, et al. (1974) report on the structural similarity of mitochondrial MDH to L-3-hydroxyacyl CoA dehydrogenase (the cytoplasmic MDH being somewhat similar to lactate dehydrogenase e). Schindler (1975) indicates differences in binding sites. Banaszak and Bradshaw (1975) have reviewed the enzymes and have compared the amino acid compositions of the two isozymes from porcine heart with those of other species. They suggest that the subforms of the isozymes that have been reported may be preparative artifacts. Applications: Suitable for use in Western Blot, Dot Blot, ELISA, Immunoperocxidase Electron Microscopy and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: ELISA: 1:1000-1:5000. Use 1ug of Malate Dehydrogenase (Porcine Heart) in a standard sandwich ELISA with ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) (A0030) as a substrate for 30 minutes at RT., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Hersteller: United States Biological
Hersteller-Nr: M2115-20

Eigenschaften

Anwendung: ELISA, Dot Blot, IHC, WB
Antikörper-Typ: Polyclonal
Konjugat: No
Wirt: Sheep
Spezies-Reaktivität: swine
Format: Highly Purified

Datenbank Information

UniProt ID : P11708 | Passende Produkte

Handhabung & Sicherheit

Lagerung: +4°C
Versand: +4°C (International: °C)
Achtung
Nur für Forschungszwecke und Laboruntersuchungen: Nicht für die Anwendung im oder am Menschen!
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