Anti-Livin (BIRC7, baculoviral IAP repeat-containing 7 (livin), KIAP, LIVIN, MLIAP, mliap, ML-IAP, l

Alternate Name: BIRC7, baculoviral IAP repeat-containing 7 (livin), KIAP, LIVIN, MLIAP, mliap, ML-IAP, livin inhibitor-of-apotosis, kidney inhibitor of apoptosis protein, melanoma inhibitor of apoptosis protein, baculoviral IAP repeat-containing protein 7, Domain Information: , Gene Ontology Terms: , Application(s): Peptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: experiments gave bands at approx 30kD and 35kD in human acute lymphoblastic leukemia MOLT4 lysate after 0.3 ug/ml antibody staining. These bands correspond to the reported isoforms with calculated MW of 32.8kD according to NP_647478.1and 30.9kD according to NP_071444.1. Recommended concentration: 0.1-1.0 ug/ml. Staining (0.3ug/ml) of MOLT4 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Peptide Blocking: Corresponding peptide is available for Peptide Blocking studies. See L3180-01A-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.