Anti-Lipocalin 2 (HNL, NGAL, Neutrophil Gelatinase Associated Lipocalin, Oncogene 24p3, Neutrophil g

NGAL is the earliest biomarker for acute kidney injury (AKI) in humans. Since the anatomy of a pig is similar to the human anatomy, pigs are widely used in medical research as models of human disease, specifically in areas like cardiology, the digestive system and transplant research. More often than ever, pigs are now being used extensively in pharmacological research, especially in toxicology studies of drug candidates. The measurement of pig NGAL promises more sensitive detection of renal injury in pigs, whether in surgical procedures or nephrotoxicity testing of drug candidates. These new NGAL antibodies will enable researchers in many fields to measure changes in NGAL levels, with the intent to determine kidney damage in pigs. NGAL is the first AKI biomarker to cover all the animal models commonly used by the pharmaceutical industry. The new pig NGAL antibody pair is expected to further strengthen NGAL's position as the preferred biomarker for nephrotoxicity studies. , Pig neutrophil gelatinase-associated lipocalin (NGAL, also called lipocalin 2 or siderocalin) may, by analogy with its homologues in other mammalian species, be released from activated neutrophils in inflammation or infection, from certain epithelial cancers, and more dramatically, from renal tubular cells in response to ischemic or nephrotoxic injury. Neutrophil gelatinase-associated lipocalin (NGAL, also called lipocalin 2, siderocalin and neutrophil lipocalin) is a member of the lipocalin family of proteins which bind and transport small lipophilic molecules. NGAL is released by activated neutrophils and occurs as 25kD glycosylated single protein chain monomers, which form dimers and small amounts of higher oligomers, as well as complexes with matrix metalloproteinase 9 (MMP-9, gelatinase B) (1). Low level expression of NGAL in a variety of epithelia may be increased in inflammation or cancers (2). The expression and release of NGAL from renal tubules are dramatically increased by ischemic or nephrotoxic injury (3). , Neutrophil Gelatinase Associated Lipocalin (NGAL) belongs to a family of lipocans which include 25 proteins (including alpha1-microglobulin and beta-lactoglobulin), which are characterized by their ability to bind small lipophilic substances in their hydrophobic core. They thereby serve as transporters of substances like retinal, biliverdins & prostaglandins. There are indications that NGAL is involved in modulation of the inflammatory response and is found in the plasma of patients after stroke. Human neutrophil gelatinase-associated lipocalin (NGAL), and its mouse analog 24p3, are members of the lipocalin family of small secreted proteins. These proteins are up-regulated in a number of pathological conditions, including cancers, and may function as transporters of essential factors. Applications: Suitable for use in ELISA, Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Matched Pair: Capture: L2499-17G, Detection: L2499-17M, Culture Medium: RPMI 1640 with 10% fetal calf serum, Hybridoma: SP2mIL6 myeloma cells with spleen cells from Female NMRIxBALB/c mice. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.