Anti-Lipocalin 2 (HNL, NGAL, Neutrophil Gelatinase Associated Lipocalin, Oncogene 24p3, Neutrophil g

Rat NGAL, so called because it is the ortholog of human neutrophil gelatinase-associated lipocalin (NGAL), is also called lipocalin 2, 25kD alpha 2-microglobulin-related protein or neu-related lipocalin. It is expressed by neutrophils and various epithelial cells, including cells of the renal proximal tubules. It is involved in physiological processes such as the binding of siderophore iron and tissue growth and regeneration. It is upregulated in a variety of pathological situations including infection, inflammation, ischemia-reperfusion and in certain adenocarcinomas. Neutrophil Gelatinase Associated Lipocalin (NGAL) belongs to a family of lipocans which include 25 proteins (including alpha1-microglobulin and beta-lactoglobulin), which are characterized by their ability to bind small lipophilic substances in their hydrophobic core. They thereby serve as transporters of substances like retinal, biliverdins & prostaglandins. There are indications that NGAL is involved in modulation of the inflammatory response and is found in the plasma of patients after stroke. Human neutrophil gelatinase-associated lipocalin (NGAL), and its mouse analog 24p3, are members of the lipocalin family of small secreted proteins. These proteins are up-regulated in a number of pathological conditions, including cancers, and may function as transporters of essential factors. , Applications: , Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA: 1:1000 (1ug/ml). NGAL as a biotinylated detection antibody forms a sandwich ELISA pair with L2499-17C (as capture antibody) for measuring rat NGAL, giving detection limit of 0.012ng/mL in an unoptimized buffer assay., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.