Anti-Leucine Rich Repeat Transmembrane Protein 2 (LRRTM2, Leucine rich repeat transmembrane neuronal

LRRTM2 (leucine rich repeat transmembrane protein # 2) is a 59kD (predicted) member of the LRRTM family of proteins. All members of this family are type I transmembrane proteins that contain 10 external LRR repeats plus a short cytoplasmic tail that binds PDF motifs. LRRTM2 is found in deep neurons of the hippocampus (CA1-CA3), dentate gyrus granule cells, and cerebellar granular cells, and in almost all peripheral tissues at some level. Mature human LRRTM2 is 483aa in length. The extracellular region contains ten LRRs (aa61-299), followed by a 73aa cytoplasmic tail (aa444-516). Over aa34-422. human LRRTM2 shares 98% aa identity with mouse LRRTM2. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~0.5ng/well., Western Blot: 1ug/ml, Immunohistochemistry: 10ug/ml detected LRRTM2 in mouse E10.5 neural tube. Cells were fixed with PBS, 4% paraformaldehyde and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100, 1% BSA. After blocking, cells were incubated with diluted F4598-39 followed by NLS557 IgG xSh in the dark. Between each step, cells were washed with PBS, BSA., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.