Anti-Interleukin 20 (IL-20)

Mature human IL-20 is a 152 aa unglycosylated protein of the IL-10 family of helical cytokines. Unlike IL-10, IL-20 functions as a monomer. Mature human and mouse IL-20 share 77% aa identity. IL-20 is produced in skin and trachea by activated keratinocytes and possibly by monocytes. IL-20 binds the IL-20 Ra-alpha/b-beta and the IL-22 R/IL-20 Rb-beta receptor complexes. IL-20 promotes the release of proinflammatory mediators in keratinocytes and is overexpressed in plaque-type psoriasis. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA: coating concentration range is 2-8ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.