Anti-Interleukin 12 (IL-12)

Interleukin-12 is produced by activated B lymphocytes and macrophages. The properties of IL-12 include the stimulation of growth of activated T lymphocytes and the development of TH1 CD4 positive cells and CD8 positive cytotoxic T cells. Applications: Suitable for use in ELISA, Western Blot and Neutralization. Other applications not tested. Recommended Dilutions: ELISA (Capture): 0.2-0.8ug/ml, Western Blot: 0.1-0.2 ug/ml. The detection limit is ~50ng/lane under non-reducing and reducing conditions. , Neutralization: The ND50 was determined to be ~0.1-0.5ug/ml in the presence of 25ng/ml of recombinant canine IL-12, using PHA activated human peripheral blood mononuclear cells (PBMCs)., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.