Anti-Interleukin-10 (IL-10)

IL-10 is a pluripotent cytokine with important immunosuppressive actions: it can inhibit cytokines involved in the Th1 response such as IL-2, Interferon gamma and also inhibits the production of pro-inflammatory cytokines such as IL-1, IL-6, IL-8 and TNF-alpha. Applications: Useful for Western Blot, immunoassays and for Neutralization of human IL-10 in bio-assays. Furthermore the antibody can be used to Immunocytochemically stain cells containing IL-10 in frozen sections as well as in cells cultured on cover slips. Recommended Dilution: Immunocytochemistry: 1:10, For neutralization of biological activity in vitro dilutions have to be made according to the amount of IL-10 to be inactivated. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Please inquire for availability of azide free solutions, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.