Anti-IL-10

The antiserum detects recombinant and native IL-10 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-10 production from fibroblasts).   In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-10 will appear due to the variable amount of glycosylation on the molecule.  The antiserum is also useful for neutralization of human of IL-10 activity in bioassays.  For neutralization, incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested.  A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended.  The antibody will not neutralize the biological activity of murine IL-10. Protein function: Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. [The UniProt Consortium]