Anti-Hyperoxidized Peroxiredoxin-3 Recombinant Antibody (Clone 5H7c)

Peroxiredoxin-3 (Prx3) is a typical 2-Cys-containing Prx that is involved in the maintenance of cellular thiol redox homeostasis. It exists as a homodimer when oxidized and is composed of an N-terminal mitochondrial targeting sequence and a catalytic domain that contains peroxidatic and resolving cysteines (Cp and Cr, respectively). Reduced Prx3 exists as a monomer and hyperoxidized Prx3 is capable of forming dodecamers. Prx3 is ubiquitously expressed and localizes to the mitochondrial matrix. During the catalytic cycle, hydrogen peroxide oxidizes Cp to form a sulfenic acid intermediate (C-SOH), which reacts with the Cr of the opposite subunit to form a disulfide bond that is subsequently restored to a reduced state by thioredoxin 2 (Trx2). In the presence of high concentrations of hydrogen peroxide, Cp is oxidized to sulfinic acid (C-SO2H), which can be reduced by sulfiredoxin (Srx), or the irreversible sulfonic acid (C-SO3H) species. Knockout of the gene encoding thioredoxin reductase 2 (TrxR2) increases the levels of SO3-Prx3 in isolated mouse embryonic endothelial progenitor cells. Hyperoxidized Prx3 (SO2/3-Prx3) is also increased during ferroptosis induced by erastin (Cay-17754) or RSL3 (Cay-19288) in vitro. Liver levels of SO2/3-Prx3 are increased in mouse models of alcoholic fatty liver disease (AFLD) and non-alcoholic fatty liver disease (NAFLD). Cayman's Hyperoxidized Peroxiredoxin-3 Recombinant Monoclonal Antibody (Clone 5H7c) can be used for ELISA and Western blot (WB) applications. The antibody recognizes hyperoxidized Prx3 at approximately 23 kDa from human samples.