Anti-Hsp70B' (Heat Shock Protein 70B', HSPA6, HSP70-6)

Cells from many organisms increase their expression of a class of proteins known as heat shock or stress proteins in response to adverse changes in their environment. The Hsp70 family of stress proteins includes a group of ~70kD highly conserved, ubiquitous proteins located within different intracellular compartments, and involved in cellular processes such as protein trafficking, folding, and prevention of aggregation. Hsp70B' is a variant of Hsp70 that originates from a separate gene, producing a protein more basic and with different stress-induction characteristics than Hsp701. The Hsp70B' gene shares 77% identity to Hsp70, and 70% identity to the constitutively expressed Hsc70. Unlike Hsp70 which shows basal levels of expression and is induced by heat and various stressors, Hsp70B' is strictly heat-inducible and shows no basal level expression2,3. , , Applications: , Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml , Immunoprecipitation: 1:100, Optimal dilutions to be determined by researcher., , Recommended Positive Controls:, HeLa Heat Shocked Cell Lysate, Hsp70B' Protein, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.