Anti-Histone H4K12Me1 Rabbit (Clone RM458)

Histone H4 is one of four core histone proteins that are involved in the organization of DNA into chromatin. Histones are globular proteins with unstructured N-terminal tails and are subject to a variety of post-translational modifications, such as methylation, acetylation, phosphorylation, and citrullination, that can influence chromatin structure and regulate gene transcription. Histone H4 can be monomethylated at lysine 12 (H4K12Me1) by Set5, which is involved in cell growth and the stress response in yeast. H4K12 is also monomethylated by N6-adenine-specific DNA methyltransferase 1 (N6AMT1) and SET domain-containing protein 6 (SETD6) in prostate cancer cells in vitro. Cayman's Histone H4K12Me1 Rabbit Monoclonal Antibody (Clone RM458) can be used for ELISA, multiplex-based assay, and Western blot (WB) applications. The antibody recognizes H4K12Me1 at 15 kDa from human and other vertebrate samples.. Cross reactivity: (+) H4K12Me1: (-) Unmodified H4K12, (-) Unmodified H4K5Me1, (-) Unmodified H4K20Me1. (+) Human: (+) Vertebrates.. Protein function: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. [The UniProt Consortium]