Anti-Histone H3K27M Rabbit - Biotinylated (Cl

Histone 3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei. It is a globular protein that contains a histone fold domain with a C-terminal alpha-helix that facilitates nucleosome interactions and chromatin compaction, as well as an unstructured N-terminal tail that extends outside of the nucleosome core, both of which are subject to various post-translational modifications (PTMs), including ubiquitination, acetylation, methylation, and phosphorylation. Histone H3 has a key role in transcriptional regulation. Expression of histone H3 containing a lysine-to-methionine substitution at residue 27 (H3K27M) is associated with tumor progression in pediatric midline gliomas.{Filbin2018} Cayman's Histone H3K27M Rabbit Monoclonal Antibody - Biotinylated (Clone RM192) can be used for ELISA, immunohistochemistry (IHC), and Western blot (WB) applications.. Cross reactivity: (+) Histone H3K27M: (-) Histone H3.. Protein function: Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. [The UniProt Consortium]