Anti-Heat Shock Protein 70 Interacting Protein (HSPBP1, Hsp70 Binding Protein)

The prokaryotic Hsp70 protein family includes DNA K, HSCA (Hsc66), and HscC (Hsc62) (1). DNAK interacts with DNAJ and GrpE heat shock protein chaperones to fold substrate proteins in an ATP-driven cycle (2). These proteins are also involved in cell protection from environmental stress and other functions which are vital for a living cell. And like eukaryotic Hsp70 homologs, DNAK participates in the assembly/disassembly of protein complexes (3, 4). , , Applications:, Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot:1:5000 , Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.