Anti-Heat Shock Protein 60 (60kD Heat Shock Protein, Mitochondrial, 60kD Chaperonin, Chaperonin 60,

A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsp's), more appropriately called as stress response proteins (srp's). hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against bacterial hsp60 and against its mammalian homolog. Applications: Suitable for use in Western Blot, Immunoprecipitation, and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 2-4ug/ml, Immunohistochemistry (paraffin): 4-8ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with 200ul sterile dH20. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.