Anti-Granzyme A (GZMA, Granzyme-1, CTL Tryptase, Cytotoxic T-lymphocyte-associated Serine Esterase 3

Granzyme A is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes and natural killer (NK) cells. Granzyme A is the most abundant protease in CTL and NK cells. It induces caspase-independent cell death when introduced into target cells by perforin. Human granzyme A is synthesized as a 262aa precursor with a signal peptide aa1-6, a propeptide aa27-28 and a mature chain aa29-262. After being activated by lysyl endopeptidase removal of the propeptide, Granzyme A cleaves thioester substrates. Mature human granzyme A shares 71% aa sequence identity with mouse granzyme A. Applications: Suitable for use in Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunocytochemistry: 5-15ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.