Anti-Glial Fibrillary Acidic Protein (GFAP)

Human GFAP is a 49.749 kD protein (432aa) expressed by astrocytes of the central nervous system. GFAP is an intermediate filament protein and acts as an intra-cellular structural component of the astrocytic cytoskeleton. During embryonic and fetal life, GFAP is also expressed by radial glial cells of the CNS. Rare mutations of the GFAP gene in humans result in Alexander's disease, one of the leukodystrophies. Applications: Suitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot. Other applications have not been tested. , Recommended Dilutions: Immunohistochemistry: 1:2000-1:5000 using 2% paraformaldehyde tissues, Immunocytochemistry: 1:2000-1:5000 using 2% paraformaldehyde cells, Western Blot: 1:10,000-1:20,000, Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1906-10 IgY, Chicken (HRP) Rb x Ch , I1906-12 IgY, Chicken (FITC) Rb x Ch , I1906-14 IgY, Chicken (AP) Rb x Ch, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.