Anti-GLG1 (CFR-1, ESL-1, MG160, MG-160, Golgi complex-Localized Glycoprotein 1, CFR1, E-Selectin lig

GLG1 (Golgi complex­Localized Glycoprotein 1, also CFR1, E­Selectin ligand­1/ESL­1, MG­160 and Cys­rich FGF receptor) is a 150­160kD (reducing, 130kD nonreducing) glycoprotein. It is expressed in both Golgi and/or the cell membrane of multiple cell types, including neutrophils (from rodents, not humans), liver stellate cells, neurons, cardiac myocytes, monocytes and bronchial epithelial cells. In the blood, GLG1/ESL­1 collaborates with PSGL­1 to mediate leukocyte binding to endothelial cell surfaces. PSGL­1 initiates leukocyte tethering while GLG1 promotes slow rolling. GLG1 also serves as an intra­Golgi receptor for multiple FGFs, including FGF­1, ­2, ­4, ­18 and possibly ­3, and as a component of an unusual latent TGF­beta complex. Mature human GLG1 is an 1150 amino acid (aa) type I transmembrane protein. It contains a 1116 aa extracellular/luminal region (aa30­1145) plus a short 13 aa cytoplasmic segment. The extracellular region possesses a 16 aa poly­Gln segment followed by 16 Cys­rich repeats (aa116­1101). There are three potential isoform variants, one of which possess a 24aa extension at the C­terminus, a second that couples the aforementioned C­terminal extension to a deletion of aa147­157, and a third that contains a 14aa substitution for aa685­1179. It is suggested that the longer C­terminus retains GLG1 in the Golgi, while shorter cytoplasmic segments allow for presentation at the cell membrane. Over aa1048­1145, human and mouse are identical in aa sequence. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.