Anti-Gephyrin (GPHN, GPH, KIAA1385)

Gephyrin (GPHN, from geoura, the Greek word for bridge? also known as molybdopterin adenyltransferase) is a 90-94kD member of both the MoaB and MoeA families of proteins. It has limited expression, being found in hepatocytes and neurons. In neurons, gephyrin is a postsynaptic density cytoplasmic protein that interacts with the subunits of glycine and GABAA receptors. This interaction is mediated by the ability of gephyrin to form hexameric lattices that act as scaffolds that link the subunits of the two inhibitory receptors to the cell's underlying tubulin network. In addition, the gephyrin lattice may also interact with NLG-1and-2, suggesting a role for gephyrin in the regulation of glutamatergic synapses. Gephyrin is also known to participate in the synthesis of molybdenum cofactor. This cofactor binds molybdenum, making it available to sulfite and aldehyde reductase. Human gephyrin is 736aa in length. It contains a 181aa N-terminal G-domain that possesses MPT Motransferase activity (aa14-166) and a homotrimer interface motif, followed by a 420aa C-terminal region (aa319-738) that contains an MPT adenyltransferase domain (aa326-736) with an embedded homodimerization motif. There are at least five utilized Ser/Thr phosphorylation sites. Multiple splice variants exist, and represent peptide insertions at multiple sites. There is a 13aa insertion after Glu98, a 33aa insertion after 243aa, and 15, 19, 21and 24aa insertions after 288aa. Over aa2-188, human and mouse Gephyrin are identical in aa sequence. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.1ug/ml, Immunohistochemistry: 5-15ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.