Anti-GALNT10 (N-Acetyl-Galactosaminyl Transferase 10, UDP-Acetylgalactosaminyltransferase 10, GalNAc

GALNT10 (N­Acetyl­Galactosaminyl Transferase 10, also UDP­Acetylgalactosaminyltransferase 10 and ppGalNAc­T10) is a member of the GalNAC transferase subfamily, glycosyltransferase 2 family of enzymes. It is widely expressed, being found in intestine, pancreas, thyroid and spleen. GALNT10 is found in the Golgi apparatus, and catalyzes the transfer of UDP­GalNAc onto either a Ser or Thr residue on a previously glycosylated peptide/polypeptide backbone. Human GALNT2 is a 603 amino acid (aa) type II transmembrane glycoprotein. It contains an 11 aa N­terminal cytoplasmic region and a 572aa extracellular domain (aa32­603). The ECD possesses two key parts, a catalytic region with two catalytic subdomains (aa144­253 and 311­373), and a ricin B­type lectin domain that binds carbohydrates (aa458­590). These two distinct domains have unique but complimentary properties. By itself, an active catalytic domain can do no more than place a GalNAc residue immediately adjacent to an existing C­terminal GalNAC residue. Alternatively, if the lectin domain is involved, this domain may recognize (bind to) any number of existing glycosylation sites, allowing for the subsequent attachment of GalNAc by the catalytic site onto a Ser or Thr residue quite distant from the initial lectin domain:CHO recognition site. There are at least three potential splice form variants. One shows a deletion of aa190­251, a second contains a 13 aa substitution for aa354­366, and a third possesses a 23aa substitution for aa1­352 coupled to a three aa substitution for Asn389. Over aa71­603, human GALNT10 shares 96% aa sequence identity with mouse GALNT10. Applications: Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5ug/ml, Immunohistochemistry: 5­15ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.