Anti-FUCA1 (Tissue alpha-L-Fucosidase)

Fucosylated glycoconjugates play numerous roles in biological processes, such as development, apoptosis and the immune responses, and are involved in the pathology of inflammation, cancer and cystic fibrosis (1, 2, 3, 4). Tissue alpha­L­Fucosidase, also known as FUCA1, is a lysosomal enzyme responsible for hydrolyzing alpha­L­fucose moieties from glycolipids and oligosaccharides (5). The mature enzyme in the liver has a molecular weight of 200kD and exists in the native state as a tetramer (6). Defects in FUCA1 are the cause of fucosidosis (7, 8, 9), an autosomal recessive lysosomal storage disease characterized by the accumulation of fucose­containing glycolipids and glycoproteins in various tissues. Serum alpha­L­fucosidase has been identified as a useful marker for oral cancer (10). Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.2ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.