Anti-Fetuin A (AHSG, Alpha-2-HS-glycoprotein, 59kD Bone Sialic Acid-containing Protein, BSP, Glycopr

Fetuin A (from the Latin word fetus for "offspring"? also a2HS [Heremans and Schmid] glycoprotein/AHSG, 59kD BSP and pp63) is a 59-63kD soluble, highly glycosylated type 3 member of the fetuin family of proteins. Originally isolated from fetal calf serum, rat fetuin A is now reported to be secreted by hepatocytes and, possibly, osteoblasts. Functionally, fetuin A appears to complex with matrix Gla protein, bind calcium phosphate and to regulate matrix mineralization. While it does not dissolve existing mineral, it does inhibit unwarranted tissue mineralization and the inflammatory reaction that accompanies it. A role for fetuin A in the regulation of insulin receptor signaling is unclear. Mature rat fetuin A is 334aa in length. It contains two cystatin fetuin A type I domains (aa19-133,144-250) that control mineralization, and a C-terminal domain (aa255-352) that may interact with the insulin receptor. There are at least four utilized phosphorylation sites at Ser138, 313, 316 and 318, and one potential internal cleavage site between Arg143-Lys144 that, if utilized, would generate a disulfide-linked heterodimer. Mature rat fetuin A shares 85% and 61% aa sequence identity with mouse and human fetuin A, respectively. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.2ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.