Anti-eNOS Antiserum

eNOS catalyzes the formation of NO from L-arginine in many cell types including vascular endothelium, bronchiolar epithelium, cardiac myocytes, spleen, and kidney. Although eNOS was initially characterized as a constitutive enzyme, expression of eNOS mRNA is altered by many factors including shear stress, fibroblast growth factor, hypoxia, and LPS. eNOS is myristoylated and palmitoylated at the N-terminus, modifications which are required for localization to the plasmalalemmal caveolae of endothelial cells. Additionally, eNOS is phosphorylated on Ser1179 by protein kinase Akt resulting in an increase in NOS activity.Synonyms: Constitutive NOS, Endothelial Nitric Oxide Synthase, Endothelial NOS, Nitric Oxide Synthase 3, cNOS. Immunogen: Synthetic peptide from the C-terminal region of human eNOS. Formulation: (Request formulation change), Polyclonal antiserum. Storage Buffer: Polyclonal antiserum when reconstituted in 100 µl of deionized water. Host: Rabbit. Applications: WB. Cross Reactivity: (-) iNOS, (-) nNOS. UniProt Accession #: P29474. Origin: Animal/Rabbit.