Anti-EGR1 (Zif268, Early Growth Response Protein 1, Krox-24, NGFI-A, TIS8, ZENK)

Early Growth Response 1, (EGR1) belongs to the EGR family of zinc finger transcription factors and regulates the expression of several tumor suppressors. Its expression is induced by diverse signals that initiate growth and differentiation. EGR1 suppresses or activates cell growth depending on the tumor type. Within the region that includes its three zinc finger domains, human EGR1 shares 99% aa sequence identity with mouse and rat EGR1. Applications: Suitable for use in Immunohistochemistry and ELISA. Other applications not tested. Recommended Dilutions: Immunohistochemistry: 8-25ug/ml using basic antigen retrieval detected EGR1 in paraffin-embedded human prostate cancer tissue sections., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.