Anti-DSC3 (Desmocollin-3, CDHF3, DSC-3, DSC4, HT-CP)

DSC­3 (Desmocollin [Greek for "glue­that­binds"]­3) is a 95­110kD member of the Ca++­dependent cadherin family of adhesion molecules. It is found on the surface of stratified epithelial cells, including keratinized and nonkeratinized epithelia of the urinary bladder, vagina, oral cavity and skin. DSC­3 serves as a component of desmosomes, forming a linkage that unites adjacent cells with cytoplasmic intermediate filaments. In particular, in the extracellular space, DSC­3 forms both homotypic and heterotypic interactions with DSG­1 in­trans, and binds to the cytoskeleton intracellularly via plakophilin­3. Mature mouse DSC­3 is a 760 amino acid (aa) type I transmembrane glycoprotein. The mature molecule contains a 560aa extracellular region with five cadherin domains (aa136­691), and a 179aa cytoplasmic domain that possesses three utilized Ser phosphorylation sites. There is one splice variant that shows an eight aa substitution for aa831­895. Over aa136­695, mouse DSC­3 shares 79% and 93% aa identity with human and rat DSC­3, respectively. Applications: Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml, Immunohistochemistry: 8­25ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.