Anti-DMPK (Myotonin-protein Kinase, MT-PK, DM-kinase, DMK, DM1 Protein Kinase, DMPK, Myotonic Dystro

DMPK also called DMK, MDPK or MT-PK is an 80kD cytoplasmic protein of the ser/thr kinase superfamily (EC 2.7.11.1). The 639aa human DMPK includes a protein kinase domain aa81-349, an AGC kinase C-terminal domain aa350-425 and a coiled-coil negative regulatory domain that mediates dimerization aa467-546. Within the region used as an immunogen, human DMPK shares 72% and 73% aa sequence identity with mouse and rat DMPK, respectively. This region is included within 12 isoforms that range in size from aa530-634. The most prominent, muscle- specific isoform phosphorylates phospholamban and is critical for cardiac contractility. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.