Anti-Diaphanous 1 (hDia1, DIAPH1, p140DIA, DFNA1, LFHL1, diaphanous homolog 1, DRF1, Diaphanous-rel

This gene is a homolog of the Drosophila diaphanous gene, and has been linked to autosomal dominant, fully penetrant, nonsyndromic sensorineural progressive low-frequency hearing loss. Actin polymerization involves proteins known to interact with diaphanous protein in Drosophila and mouse. It has therefore been speculated that this gene may have a role in the regulation of actin polymerization in hair cells of the inner ear. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. Applications: Suitable for use in ELISA and Western Blot. Other applications have not been tested. , Recommended Dilutions: Peptide ELISA Titer: 1:32,000, Western Blot: 0.3-1ug/ml ~140kD band observed in lysates of cell line NIH3T3 (calculated MW of 141kD according to Human NP_005210.3 and 139kD according to Mouse NP_031884.1). , Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.