Anti-DAP3 (Death Associated Protein 3)

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that also participates in apoptotic pathways which are initiated by tumor necrosis factor-alpha, Fas ligand, and gamma interferon. This protein potentially binds ATP/GTP and might be a functional partner of the mitoribosomal protein S27. Splice variants that differ in the 5' UTR have been found for this gene, both variants encode the same protein. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q. Applications: Suitable for use in ELISA, Western Blot, Immunofluorescence, Immunocytochemistry and Immunohistochemistry. Other applications have not been tested. , Recommended Dilutions: Peptide ELISA Titer: 1:16,000, Western Blot: 1-3ug/ml ~40kD band observed in lysates of cell line HeLa (calculated MW of 45.6kD according to NP_387506.1). In transfected HEK293 transiently expressing DAP3 a band of approx. 48kD is observed. This band is not observed in the non-transfected HEK293., Immunohistochemistry: 2.5ug/ml, paraffin embedded Human Tonsil and Brain (Cortex). , Immunocytochemistry: 3-10ug/ml, mitochondrial staining of HeLa after in situ denaturation of the target protein using 3-4M guanidinium thiocyanate The signal goes down upon knockdown of the DAP3 expression., Immunofluorescence: 5ug/ml., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.