Anti-Contactin-1 (Neural Cell Surface Protein F3, Cntn1)

CNTN1 (Contactin1? also neuronal cell surface protein F3/F11) is a 135-145kD GPI-linked glycoprotein member of the Contactin family, Ig superfamily of molecules. It is expressed on the surface of oligodendrocytes and neurons, principally on axons, and has multiple binding partners. In particular, it binds to astrocyte tenascin C, Schwann cell NF/neurofascin155, and oligodendrocyte produced tenascin-R and Notch intrans, plus paranodin in cis. Tenascin-R interactions inhibit neurite outgrowth, paranodin/NF155 interactions may contribute to Ranvier node development, and Notch ligation results in oligodendrocyte maturation. CNTN1 also binds to chondroitin sulfate E, an interaction that initiates neurite outgrowth. Mouse CNTN1 is synthesized as a 1020aa preproprecursor (aa1-1020). It contains a 20aa signal sequence, followed by a 981aa mature region (aa21-1001), and a 19aa propeptide. The mature region possesses six consecutive C2-type Ig-like domains (aa41-603) that regulate neurite outgrowth, followed by three FN-type III domains (aa605-901) that regulate adhesiveness. It is estimated that 15% of the native MW constitutes carbohydrate, part of which is represented by the HNK 1 antigen (3sulfo-GluA-Gal-GlcNac). Over aa20-999, mouse CNTN1 shares 96% and 99% aa sequence identity with human and rat CNTN1, respectively. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.2ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.