Anti-Connexin 43 (Gap Junction alpha-1 Protein, GJA1, CX43, GJAL, ODDD, ODOD, SDTY3) (APC)

Connexin 43 (Cx43, also gap junction alpha-1 protein) is a 41-44kD member of the connexin family, alpha-type subfamily, of transmembrane proteins. It is the most common type of connexin in cardiac muscle cells, and also occurs in hepatocytes, astrocytes, and ovary granulosa cells. It is a four transmembrane protein that is 382 amino acids (aa) in length and contains two cytoplasmic tails. One is 12 aa in length at the N-terminus and the second is 151 aa in length at the C-terminus. Human and rat Cx43 share 98% aa sequence identity over the entire length of the molecule. Applications: Suitable for use in ELISA and Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ul/10e6 cells using HEK293 human embryonic kidney cell line transfected with human Connexin 43/GJA1 and eGFP., Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.